ENZYMATIC SYNTHESIS OF NUCLEOSIDE DIPHOSPHATES AND TRIPHOSPHATES
نویسندگان
چکیده
منابع مشابه
Enzymatic synthesis of nucleoside diphosphates and triphosphates.
It has been recognized from recent studies that nucleoside triphosphates, other than ATP,’ function in biologic systems as phosphorylating agents (1) and in coenzyme synthesis (2) in a manner analogous to that of ATP. The discovery of the natural occurrence of a variety of purine and pyrimidine nucleoside diand triphosphates (3-5) and the likelihood that new biologic functions for these nucleot...
متن کاملEnzymatic synthesis of nucleoside S-mono and -triphosphates
Recently we have reported on the chemical synthesis of dNTP(3’F) and NTP(3’F) and their use as terminators of the biosynthesis of DNA and RNA, respectively [l-4]. During the course of the chemical synthesis of fluorodeoxyriboand 2 ’ -deoxyribo-NMP and NTP, we have pointed out that the isolated yields of pure compounds are varied within the broad limits depending on the structure of a heterocycl...
متن کاملNucleoside triphosphates--from synthesis to biochemical characterization.
The traditional strategy for the introduction of chemical functionalities is the use of solid-phase synthesis by appending suitably modified phosphoramidite precursors to the nascent chain. However, the conditions used during the synthesis and the restriction to rather short sequences hamper the applicability of this methodology. On the other hand, modified nucleoside triphosphates are activate...
متن کاملEnzymatic Primer-Extension with Glycerol-Nucleoside Triphosphates on DNA Templates
BACKGROUND Glycerol nucleic acid (GNA) has an acyclic phosphoglycerol backbone repeat-unit, but forms stable duplexes based on Watson-Crick base-pairing. Because of its structural simplicity, GNA is of particular interest with respect to the possibility of evolving functional polymers by in vitro selection. Template-dependent GNA synthesis is essential to any GNA-based selection system. PRINC...
متن کاملGlycerol nucleoside triphosphates: synthesis and polymerase substrate activities.
[Structure: see text] The synthesis of (S)-glycerol nucleoside triphosphates (gNTPs) and the analysis of their substrate activities for enzymatic polymerization is described. NTPs with simplified carbohydrate backbones such as the tNTPs (alpha-L-threose-NTPs) are polymerase substrates and offer the potential to create non-natural aptamer sequences with simplified backbones through enzymatic mea...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1955
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)66050-8